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Registro Completo |
Biblioteca(s): |
Embrapa Semiárido. |
Data corrente: |
17/10/2012 |
Data da última atualização: |
16/09/2022 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
SCHERER, R.; RYBKA, A. C. P.; BALLUS, C. A.; MEINHART, A. D.; TEIXEIRA FILHO, J.; GODOY, H. T. |
Afiliação: |
RODRIGO SCHERER; ANA CECILIA POLONI RYBKA, CPATSA; CRISTIANO AUGUSTO BALLUS; ADRIANA DILLENBURG MEINHART; JOSÉ TEIXEIRA FILHO; HELENA TEIXEIRA GODOY. |
Título: |
Validation of a HPLC method for simultaneous determination of main organic acids in fruits and juices. |
Ano de publicação: |
2012 |
Fonte/Imprenta: |
Food Chemistry, v. 135, p. 150-154, 2012. |
Idioma: |
Inglês |
Conteúdo: |
A liquid chromatographic method for fast and simultaneous determination of tartaric, malic, ascorbic and citric acids was validated for further application to fruits and juices. Moreover, the organic acids content of commercial samples of fruits and juices were evaluated, as well as the ascorbic acid stability during the storage. Determination of organic acids was carried out using a liquid chromatograph coupled to a diode array detector, with reversed phase (C18 column) and isocratic elution with 0.01 mol L1 KH2PO4 (pH = 2.60) mobile phase. The validation parameters showed efficiency, adequate linearity, relative standard deviation values between 0.4% and 2.3% (n = 10) for repeatability and from 1.2% to 5.0% (n = 18) for reproducibility, limits of detection (LD) were between 0.03 and 3.31 lg mL1 and quantification (LQ) were between 0.10 and 11.03 lg mL1, recovery rates were between 82% and 110%, for two levels. In addition, the method is fast (10 min) and generates low and non-toxic residues. The values found for vitamin C were about 10 times above the values declared at the package. Ready to drink juices have a composition similar to the fruit, concerning to organic acids, except for the powder juice, in which only ascorbic and citric acids were found, for all tastes. After opening the package, a decrease of 14.0% and 27.0% in ascorbic acid content was observed for orange powder and ready to drink juices, respectively. |
Palavras-Chave: |
Ácidos orgânicos; Cromatografia líquida; Estabilidade de ácido ascórbico; HPLC; Sucos de frutas; Validação do método. |
Thesagro: |
Fruta; Pós-Colheita; Processamento; Suco. |
Thesaurus Nal: |
Fruits; Juices. |
Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/68195/1/Artigo-Ana-Cecilia-2012-1.pdf
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Marc: |
LEADER 02355naa a2200325 a 4500 001 1936986 005 2022-09-16 008 2012 bl uuuu u00u1 u #d 100 1 $aSCHERER, R. 245 $aValidation of a HPLC method for simultaneous determination of main organic acids in fruits and juices.$h[electronic resource] 260 $c2012 520 $aA liquid chromatographic method for fast and simultaneous determination of tartaric, malic, ascorbic and citric acids was validated for further application to fruits and juices. Moreover, the organic acids content of commercial samples of fruits and juices were evaluated, as well as the ascorbic acid stability during the storage. Determination of organic acids was carried out using a liquid chromatograph coupled to a diode array detector, with reversed phase (C18 column) and isocratic elution with 0.01 mol L1 KH2PO4 (pH = 2.60) mobile phase. The validation parameters showed efficiency, adequate linearity, relative standard deviation values between 0.4% and 2.3% (n = 10) for repeatability and from 1.2% to 5.0% (n = 18) for reproducibility, limits of detection (LD) were between 0.03 and 3.31 lg mL1 and quantification (LQ) were between 0.10 and 11.03 lg mL1, recovery rates were between 82% and 110%, for two levels. In addition, the method is fast (10 min) and generates low and non-toxic residues. The values found for vitamin C were about 10 times above the values declared at the package. Ready to drink juices have a composition similar to the fruit, concerning to organic acids, except for the powder juice, in which only ascorbic and citric acids were found, for all tastes. After opening the package, a decrease of 14.0% and 27.0% in ascorbic acid content was observed for orange powder and ready to drink juices, respectively. 650 $aFruits 650 $aJuices 650 $aFruta 650 $aPós-Colheita 650 $aProcessamento 650 $aSuco 653 $aÁcidos orgânicos 653 $aCromatografia líquida 653 $aEstabilidade de ácido ascórbico 653 $aHPLC 653 $aSucos de frutas 653 $aValidação do método 700 1 $aRYBKA, A. C. P. 700 1 $aBALLUS, C. A. 700 1 $aMEINHART, A. D. 700 1 $aTEIXEIRA FILHO, J. 700 1 $aGODOY, H. T. 773 $tFood Chemistry$gv. 135, p. 150-154, 2012.
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Registro original: |
Embrapa Semiárido (CPATSA) |
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Registro Completo
Biblioteca(s): |
Embrapa Rondônia. |
Data corrente: |
20/01/1997 |
Data da última atualização: |
14/05/2013 |
Autoria: |
OLIVEIRA, F. N. S.; LIMA, J. A. de S. |
Título: |
Introducao e avaliacao de cultivares de mandioca (Manihot esculenta Crantz) em Rondonia. |
Ano de publicação: |
1986 |
Fonte/Imprenta: |
Porto Velho: EMBRAPA-UEPAE Porto Velho, 1986. |
Páginas: |
6p. |
Série: |
(EMBRAPA-UEPAE Porto Velho. Pesquisa em Andamento, 99). |
ISSN: |
0101-7039 |
Idioma: |
Português |
Conteúdo: |
Este trabalho objetiva identificar cultivares de mandioca portadoras de características superiores, que apresentem boa produção de raízes e que melhor se adaptem às condições ambientais de Rondônia. |
Palavras-Chave: |
Avaliacao; Brasil; Cultivar; Cultivate; Evaluation; Introdução; Rondônia; Variedades; Variety. |
Thesagro: |
Mandioca; Manihot Esculenta; Variedade. |
Thesaurus NAL: |
Brazil; cassava. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/82791/1/FOL-3755-0001.pdf
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Marc: |
LEADER 01063nam a2200325 a 4500 001 1698599 005 2013-05-14 008 1986 bl uuuu u0uu1 u #d 022 $a0101-7039 100 1 $aOLIVEIRA, F. N. S. 245 $aIntroducao e avaliacao de cultivares de mandioca (Manihot esculenta Crantz) em Rondonia. 260 $aPorto Velho: EMBRAPA-UEPAE Porto Velho$c1986 300 $a6p. 490 $a(EMBRAPA-UEPAE Porto Velho. Pesquisa em Andamento, 99). 520 $aEste trabalho objetiva identificar cultivares de mandioca portadoras de características superiores, que apresentem boa produção de raízes e que melhor se adaptem às condições ambientais de Rondônia. 650 $aBrazil 650 $acassava 650 $aMandioca 650 $aManihot Esculenta 650 $aVariedade 653 $aAvaliacao 653 $aBrasil 653 $aCultivar 653 $aCultivate 653 $aEvaluation 653 $aIntrodução 653 $aRondônia 653 $aVariedades 653 $aVariety 700 1 $aLIMA, J. A. de S.
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